Job Title Senior Scientist
Duration: 6 months + with possible extension
Site: Cambridge, MA
The scientist will be responsible to run in vitro primary cell culture in 96-well plate format, perform serial dilutions of drug molecules, perform ELISA for cytokine analysis, and perform FACS flow cytometry analysis to look at cell populations changes and cell phenotype changes. Candidates with previous mRNA LNP production and characterization, and LNP antibody conjugation are preferred.
1. In vitro primary cell culture (human PBMC, Cyno PBMC, and mouse splenocyte)
2. Serial dilutions of drug molecules and application of treatment using multichannel pipette
3. Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
4. Good to have: mRNA LNP production and characterization
5. Good to have: LNP antibody conjugation
3-5 year experience level
Masters preferred
1st Round 30 minutes MS Teams with sponsor
2nd Round 30 minute MS Teams with team or onsite
EEO:
Mindlance is an Equal Opportunity Employer and does not discriminate in employment on the basis of – Minority/Gender/Disability/Religion/LGBTQI/Age/Veterans